Probing Protein Interactions of Cyanylated Acyl Carrier Proteins using Vibrational Spectroscopy

Correction to “Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy”

■ REFERENCES (1) Corrected ref 2: Nguyen, C.; Haushalter, R. W.; Lee, D. J.; Markwick, P. R. L.; Bruegger, J.; Caldara-Festin, G.; Finzel, K.; Jackson, D. R.; Ishikawa, F.; O’Dowd, B.; McCammon, J. A.; Opella, S. J.; Tsai, S.-C.; Burkart, M. D. Nature 2014, 505, 427. (2) Corrected ref 3: Masoudi, A.; Raetz, C. R. H.; Zhou, P.; Pemble, C. W., IV. Nature 2014, 505, 422. (3) Corrected ref 4: Dutta...

Probing the Phosphopantetheine Arm Conformations of Acyl Carrier Proteins Using Vibrational Spectroscopy

Acyl carrier proteins (ACPs) are universal and highly conserved domains central to both fatty acid and polyketide biosynthesis. These proteins tether reactive acyl intermediates with a swinging 4'-phosphopantetheine (Ppant) arm and interact with a suite of catalytic partners during chain transport and elongation while stabilizing the growing chain throughout the biosynthetic pathway. The flexib...

Single Molecule Probing of Exocytotic Protein Interactions Using Force Spectroscopy.

Relatively recently, the Atomic Force Microscope (AFM) emerged as a powerful tool for single molecule nanomechanical investigations. Parameters that can be measured by force spectroscopy using AFM, such as the force and total mechanical extension required to break bonds between various proteins can yield valuable insights into the nature of the bond (zippering vs. highly localized binding site)...

Versatility of acyl-acyl carrier protein synthetases.

The acyl carrier protein (ACP) requires posttranslational modification with a 4'-phosphopantetheine arm for activity, and this thiol-terminated modification carries cargo between enzymes in ACP-dependent metabolic pathways. We show that acyl-ACP synthetases (AasSs) from different organisms are able to load even, odd, and unnatural fatty acids onto E. coli ACP in vitro. Vibrio harveyi AasS not o...

Probing Biomolecular Interactions Using Nanopore Force Spectroscopy